0.1 M Sodium Phosphate Buffer

Recipe

Sodium phosphate

1 1000 sodium phosphate buffer (pH six.0–7.2)

Mixing 1 One thousand NaH₂PO₄ (monobasic) and 1 G Na₂HPO_four (dibasic) stock solutions in the volumes designated in the tabular array below results in 1 L of 1 M sodium phosphate buffer of the desired pH. To prepare the stock solutions, dissolve 138 yard of NaH₂PO₄•H_iiO (monobasic; m.w. = 138) in sufficient H₂O to brand a concluding book of 1 L and dissolve 142 1000 of Na₂HPO_four (dibasic; grand.westward. = 142) in sufficient H₂O to make a final volume of 1 Fifty.

Volume (mL) of 1 G NaHiiPO4	Volume (mL) of i M NatwoHPO4	Concluding pH
877	123	six.0
850	150	6.1
815	185	6.2
775	225	6.iii
735	265	half-dozen.iv
685	315	six.v
625	375	6.six
565	435	6.7
510	490	6.8
450	550	6.ix
390	610	7.0
330	670	7.1
280	720	7.2

0.1 M sodium phosphate buffer (pH 7.4)

Add 3.1 g of NaH_iiPO_iv•H₂O and 10.9 g of Na₂HPO₄ (anhydrous) to distilled H_twoO to brand a volume of 1 L. The pH of the final solution will exist 7.4. This buffer tin can exist stored for up to 1 mo at 4°C.

0.1 M sodium phosphate buffer (from 1 One thousand stocks) at 25°C

To prepare 1 L of 0.i M sodium phosphate buffer of the desired pH, the post-obit mixtures should be diluted to one L (last volume) with H₂O.

pH	Volume (mL) of 1 M Na2HPO4	Volume (mL) of 1 K NaHiiPO4
five.8	7.9	92.i
vi.0	12.0	88.0
6.2	17.eight	82.2
six.four	25.5	74.5
6.6	35.2	64.8
6.8	46.3	53.7
7.0	57.7	42.3
seven.two	68.4	31.6
seven.4	77.iv	22.6
7.6	84.v	fifteen.5
7.8	89.vi	10.iv
8.0	93.ii	6.8

• © 2006 Cold Spring Harbor Laboratory Printing

• 

0.1 M Sodium Phosphate Buffer,

Source: http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8303

Posted by: gonzalezcommens.blogspot.com

Share this post